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Serodetection of btb using multiantigens
Journal
Diagnostic
Microbiology and Infectious Disease -
Vol
46, Iss3 , July 2003, pp197-203
Authors
Rena Greenwald, Javan Esfandiari, Sandrine Lesellier, Raymond Houghton, John Pollock, Claus Aagaard, Peter Andersen, R. Glyn Hewinson, Mark
Chambers, and Konstantin Lyashchenko from the Chembio Diagnostic Systems, Inc., USA;
Veterinary Laboratories Agency, Surrey; Corixa Corporation, Washington, USA;
Department of Agriculture and Rural Development, Belfast; and the Department of TB Immunology, Copenhagen, Denmark.
Abstract
Despite attempts to control bovine tuberculosis, the incidence of disease in
Great Britain continues to rise. In GB, the European badger (Meles meles) is a
reservoir of infection with Mycobacterium bovis. In an effort to improve the
serodetection of badger tuberculosis, we examined sera from M. bovis
culture-positive and culture-negative badgers for their ability to recognize M.
bovis antigens, using a multi-antigen print immunoassay (MAPIA). Depending on
the antigens used in the MAPIA, the assay had a sensitivity of 49–59% and a
specificity of 84-88% Results from the MAPIA were used to select antigens for
the development of a lateral-flow immunoassay. This so-called ‘Rapid Test’ used
5μl of serum and gave unambiguous results within 10 min. When applied to 178
badger sera, the Rapid Test had a sensitivity of 53% and a specificity of 95%.
This represented an improvement over the performance of the existing ELISA Test,
which had a sensitivity of 47% and a specificity of 89% on the same sera. This
is the first report of a diagnostic test for badger tuberculosis that can be
performed alongside the captive animal.
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